Alcoholx Show full post »
Alcoholx
so the wlp775 yeast is now in the freezer..  a pretty simple process "if" it works, i boiled a mason jar and any utensils i was going to use.. including the 50ml centrifuge tubes, mixed 50ml of glycerin with 50ml of water into the mason jar and put the lid on and boiled the whole jar containing the mixture to sanitize...

then chilled it down in cold water in the sink, with the intention of splitting that into 4 vials..  so 25ml of yeast and 25ml of solution to give me 25% glycerin mix.

there was not as much yeast as i expected it had compacted down quite a lot but i drained off the apple juice and gave it a swirl..  i made a bit of a mistake here because there was too much juice still in there so it was a bit runny but i decided just to crack on...  there was only enough yeast to fill 3 tubes too 25ml.. then i filled them up to 50ml with the glycerin mixture.. chilled them in the fridge for a few hours then put them in a freezer bag and stuck them in the freezer stood up, checked them the next day and they were frozen.. so i guess only time will tell now, i think i will leave it a month then try and make a starter out of one of the vials to see if it has survived.. 

i am going to start harvesting all the yeasts i use from now on and record what generation they are ect.. i should easily get enough yeast to fill a mason jar to keep in the fridge and 4 tubes or so frozen in the freezer from an initial batch of beer..

pics!..

IMG_20200426_211436284.jpg  IMG_20200426_212210394.jpg  IMG_20200426_213827191.jpg  IMG_20200427_172024500.jpg  IMG_20200427_172051004.jpg
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Clibit
Kudos.  👍

I'm always impressed by people doing yeast stuff. 
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EspeciallyBitter
Great write up, Alco. 👍

What's your plan for using these frozen cultures, are you just going to thaw a whole tube and pitch the whole thing into a starter, or will you use a sterile loop and plate a sample and take a colony from there to build a starter?
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Alcoholx
cheers EB,  i will just make a starter from the vials i recon..  i think there will be 2 things i can do, if i make an oversize starter i could just harvest yeast from the starter and fill another tube before it gets used in a batch of beer, then use the rest of the starter as per normal or just build a starter and re-use/wash the yeast cake and store it in mason jars until that generation from a vial becomes defunct.. although in theory i could freeze a 2nd-3rd-4th ect generation of yeast and do the whole process again so i guess you could get years of yeast from your single batch of beer.. 

the idea of having lots of yeast available really appeals to me, its cheap to do as i baught 50 vials for £17 and 1ltr of gelatin was about £8 so i guess it will save money long term but just the idea that if the homebrew shops are out of stock it wont matter because i have it in the freezer is a big bonus i recon, plus it just opens up the choice of what i can do going forward.
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Alcoholx
looking to make up a cider next week as the fridge will be taken up with a lager for a month..   so i pulled a vial of the wlp 775 out the freezer

quick thawed it in 25-30c water as it seems this is the way to do it apparently..   put it in 500ml of apple juice and left it for a few hours on the stir plate and it seems to be alive and well..  so i have added the other half of the carton of juice and will leave it for a few days doing its thing

while the yeast has only been in the freezer for a month..  its behaved like it was never in there, picking up exactly where it left off after it had been revived after an 18 month stint in the fridge..   so if this knakerd old yeast can survive then the fresh ale yeasts i have frozen should be in excellent shape i would of thought..

while i had the pans and jars out ect for sanitising i put the bry 97 i had top cropped into 3 new vials..  had a nice thick yeast in each vial so should hopefully be a good yeast count..

feeling good about this process..  it seems that its going to work quite well and use very little space, happy days

more pictures...  i like pictures 😃

IMG_20200522_153658553.jpg  IMG_20200522_154037824.jpg  IMG_20200522_154744041.jpg  IMG_20200522_155511025_BURST000_COVER.jpg      IMG_20200522_160158886.jpgIMG_20200522_162103114.jpg IMG_20200522_194630936.jpg
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EspeciallyBitter
Excellent news! 👏

Other than BRY-97 do you have any ale yeasts already frozen?
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Clibit
Great work Alco.  👍
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GHW
I really ought to do more like this. My yeast ranching extends to keeping jars of slurry in the fridge.
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Alcoholx
Excellent news! 👏

Other than BRY-97 do you have any ale yeasts already frozen?


only the hog norsk so far pal..  i top cropped that and filled 3 vials the same as the bry , i plan to store all the yeasts i use if i can as it just seems daft not too.. i probably have 8 different dry yeasts in the fridge that i am going to slowly get through.. there not expensive strains but there all good ones.. ie: windsor and kviek, nottingham ect

the cider yeast is doing really well now.. looking spot on considering i only pitched it this afternoon.. 







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Alcoholx
IMG_20200523_064525013.jpg 

i recon thats a winner..  👍
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Pinto
Im thinking that you have to do some pretty extreme things to it to actually kill yeast off completely tbh - but I guess thats why it's flourished for millions of years in the wild :
Beer is like porn - you can buy it easily enough, but its so much more fun to make it [wink]
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