HisDudeness Show full post »
justbeaz
Fermented Culture wrote:

You could just collect trub or krausen and put it in a jar in the fridge or add it with some glycerine and freeze it. Here's quite a good video, but you don't need add the water:



Also a decent article:

http://www.woodlandbrew.com/2013/01/yeast-storage.html



intresting there F C thanks for the post always thought could you freeze yeast and would it be usable and now i know.
I like my water with barley and hops
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Pesho77

 Ive tried both of these methods and I'm getting poor results from both, I think the slants ( I use plates not tubes) got too cold in my fridge and froze so I think there all ruined, ill test one and see, but the freezing in glycerol isn't working every time either, the reason I got in to yeast banking if because my supplier (themaltmilller.co.uk) rarely has the yeasts I want, so I figured id keep my own.

 Is any one finding consistent results with their yeast bank ?

 Pesh 
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Simonh82
Pesho77 wrote:

 Ive tried both of these methods and I'm getting poor results from both, I think the slants ( I use plates not tubes) got too cold in my fridge and froze so I think there all ruined, ill test one and see, but the freezing in glycerol isn't working every time either, the reason I got in to yeast banking if because my supplier (themaltmilller.co.uk) rarely has the yeasts I want, so I figured id keep my own.

 Is any one finding consistent results with their yeast bank ?

 Pesh 


I believe plates aren't ideal for long term storage as they have a tendency to dry out and crack.  I would still test whether your yeast is viable if you think they've been frozen you may be lucky.  You might want to nudge the setting on your fridge up a bit so they aren't freezing though.

I've not used the glycol method personally but I've read about it and it seems pretty reliable.  What's the actual problem you are having with it?  Have you tried to revive any of the glycol stored yeast yet?  Did you just take a tiny bit to build up from, or did you thaw out the whole tube?

Also, just to say, storing on slants can work well.  I revived a year old slant with no problem recently. 
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HisDudeness
Yeah getting good results with my slants, I have had a couple get frozen which ruined them when I left them too close to back of fridge. Luckily those were just blanks, I keep the inoculated ones in the door shelves near the top and they store fine
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Pesho77
 Yeah the plates was probably my fault I have a fridge set up with a inkbird contoler it was set to 2c but this seams too low, ive  cranked it up to 4c and well see what happens next time, I do want to get this right so I'm not relying on suppliers.

 The glycol ones could have been too weak to survive freezing ( my best guess) but it seams they either work or don't.

 my method is to pour off as much clear liquid as I can then use the rest, I keep 10ml tubes so there's only 5ml of yeast (could be worth a try with bigger tubes maybe 100ml) then make up 1Ltr with 60g DME and some yeast nutrient, then I take 100ml of this and add the yeast,. I would expect to see some activity after 6 hours or so (some thing else to try would be 100g dme).

 So stuff to try to improve my yeast bank.

 Pesh
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